The NIH Violence Research Initiative details research opportunities that the National Institutes of Health have conducted on firearm injury and mortality prevention. The projects, funded through annual Congressional appropriations of $12.5 million, study the underlying causes and evidence-based methods of prevention of firearm injury and mortality, including crime, from a public health approach. This resource demonstrates how these critical investments have helped evolve the research field. In addition to funding innovative research projects (FY2020, FY2021, FY 2022, FY2023), NIH has also developed coordinating networks to improve evaluations and enhance the impact and generalizability of study findings. Additionally, NIH has expanded the field of qualified researchers, similar to CDC’s investments in early career researchers beginning work in this field.